Detail Article

Informations générales (source: ClinicalTrials.gov)

Numéro NCT

NCT02354079 Active, sans recrutement

Titre

HYPOCHOL : A Genetically-based Strategy to Identify New Targets in Cholesterol Metabolism (HYPOCHOL)

Type

Interventional

Pathologie

Hypobêtalipoprotéinémies

Phase

N/A

Promoteur

Nantes University Hospital (Voir sur ClinicalTrials)

Date de début

janvier 2016

Date de fin

janvier 2032

Dernière mise à jour

29 juin 2024

Résumé

The aim of this study is to identify new targets in cholesterol metabolism thanks to a genetically-based strategy.

Détail

Voir le détail The goal is to recruit 400 subjects: 200 adult subjects with familial hypobetalipoproteinemia (FHBL) (index cases) plus 200 additional related subjects in at least 10 large informative FHBL families, in which there is no known mutation in FHBL genes. The patient care is modified: patient will have an Hospital Anxiety and Depression (HAD) questionnaire (focus on depressive syndrome), a food diary, and some additional blood analysis (including genetic analysis). One of the main issue to recruit FHBL patients is the fact that they are asymptomatic and that FHBL is not identified as a serious illness by their general physicians. Step-1. Excluding mutations in selected candidate genes As a first approach to screen candidate genes and exclude patients with known mutations, the investigators developed a custom design based on the Haloplex™ technology (Agilent® Technologies) to perform high-throughput sequencing of the coding regions of 10 genes, including those previously described in FHBL (apolipoprotein B (APOB), Proprotein convertase subtilisin/kexin type 9 (PCSK9)), Microsomal triglyceride transfer protein (MTP or ABL), chylomicron retention disease (CMRD), Secretion associated, Ras related GTPase (SARA2 gene), as well as 6 additional candidate genes in cholesterol metabolism (low density lipoprotein receptor (LDLR), Sortilin (SORT1), Inducible Degrader of the LDL receptor (IDOL), Cholesteryl ester transfer protein (CETP), Apolipoprotein E (ApoE) and Angiopoietin-like Protein 3 (ANGTPL3)). All the recruited index cases (n=200) will be genotyped to select only those without mutations in previously described genes, being approximately 50% of our index case cohort. Step-2. Identification of informative families and exome sequencing In patients without identified mutations, the investigators will conduct a familial screening in order to identify other cases of FHBL among proband relatives. An analysis of fasting plasma lipid parameters (total cholesterol (TC), High density lipoprotein cholesterol (HDL-C), Low-Density Lipoproteins (LDL-C) and triglycerides (TG)) will be performed for each related. Affected subjects will be determined by a spontaneous LDL-C < 80 mg/dl and/or apoB < 50 mg/dl. In contrast, non-affected subjects will display LDL-C > 80 mg/dl and/or apoB > 50 mg/dl. For large families, the investigators will then combine whole-exome sequencing and linkage analysis to identify any novel genetic variant likely explaining FHBL. Depending on family pedigree, whole-exome sequencing (WES) will be performed on 2 to 5 patients per family. All relatives will be genotyped for linkage analysis. In parallel to this genetic approach, a regional epidemiological analysis will be performed to identify some geographical clusters with a high prevalence of the disease, as developed in the project named VaCaRMe (for Vascular and Cardiac, Respiratory and Metabolic overcome diseases) An additional aim, based on an exhaustive phenotyping of FHBL patients, is to investigate the safety of very low LDL-C and to perform some genotype-phenotype correlations in patients with FHBL population." Fermer le détail

Etablissements

Les établissements sans correspondance certaine dans le répertoire FINESS dont les données sont issues de ClinicalTrials.gov Origine et niveau de fiabilité des données
CHU de Nantes - 44093 - Nantes - France				Contact (sur clinicalTrials)

Critères

Genre

Tous

Nombre d'inclusion

Critère d'inclusion

Inclusion Criteria:

- For index cases without family screening:

o Patient with HBL: fasting LDL-C ≤ 50 mg/dl.

- For familial affected cases:

- Relative with HBL: fasting LDL-C ≤ 80 mg/dl and/or Apo B ≤ 50 mg/dl and at
least one related family case suffering from HBL.

All subjects, including familial non-affected cases, must give written consent (dated and
signed) to participate at the constitution of biobank (including DNA samples and urine
samples).

Critère de non inclusion

- Use of lipid-lowering drugs (statins, fibrates, ezetimibe, bile-acid sequestering
resin) or nutraceuticals known to affect lipids (red yeast rice, margarine and dairy
with plant sterol)

- Patient screened within an extreme metabolic disturbance (emergency situations,
sepsis, hospitalization in intensive care unit)

- Patients with hyperthyroidism, severe liver failure, end stage chronic kidney
disease, serious pancreatic failure, anemia related to thalassemia or sickle cell
disease, strict vegan diet or malnutrition

- Refusal of the patient or his legal representative to participate in the study"

NCT02354079 - HYPOCHOL : A Genetically-based Strategy to Identify New Targets in Cholesterol Metabolism (HYPOCHOL)

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Etablissements
Critères
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