Informations générales (source: ClinicalTrials.gov)
A Phase I - II Study to Assess Safety and Efficacy of the Combination of Ponatinib With High or Intermediate-Dose Cytarabine as Consolidation Therapy for Patients With Intermediate-Risk Cytogenetic FLT3-ITD AML iIn First Complete Remission (PONATINIB-AML)
Interventional
Phase 1/Phase 2
Versailles Hospital (Voir sur ClinicalTrials)
juillet 2013
novembre 2023
29 juin 2024
This project is part of a joint ALFA and GOELAM strategy aiming to improve the survival
of patients with newly diagnosed Acute Myeloid Leukemia (AML) aged 18-70 years. The basis
of this strategy is to evaluate intensified conventional chemotherapy and targeted drugs
in selected disease-risk subgroups of adult patients with non promyelocytic AML.
Participation will be proposed to almost all adult patients in France aged 18-70 years
and diagnosed with AML.
FLT3 genetic alterations include FLT3 somatic point mutations within the second tyrosine
kinase domain and internal duplications of the juxta-membrane domain. This alteration is
refered to as FLT3-ITD. The FLT3-ITD mutation is found in around 30% of patients with
cytogenetically normal AML. Patients with the FLT3-ITD genotype have been reported to
have a poor outcome when treated with conventional chemotherapy with an estimated 4-year
relapse-free survival of 25% (Schlenk et al. N Engl J Med 2008). More recently, the
prognostic relevance of FLT3-ITD has been studied in the context of integrated genetic
profiling. This confirmed the genetic complexity of AML and also that FLT3-ITD was
associated with reduced overall survival in intermediate-risk AML. A multivariate
analysis of several genetic alterations revealed that FLT3-ITD was the primary predictor
of patient outcome. FLT3-ITD mutations were classified in 3 categories: 1) FLT3-ITD with
+8, TET2, DNMT3A or MLL-PTD mutations (3-year OS 14.5%); 2) FLT3-ITD with wild type
CEBPA, TET2, DNMT3 and MLL-PTD (3-year OS 35.2%) and 3) FLT3-ITD with CEBPA mutations
(3-year OS 42%) (Patel JP et al. N Engl J Med 2012). However, FLT3-ITD was not a
predictor of response to induction therapy, allowing the introduction of targeted
therapies after the induction course.
Several FLT3 inhibitors have been evaluated or are currently being tested in the setting
of relapsing AML. In most trials to date, patients were only eligible if the FLT3-ITD
mutation was present. Disappointing results were reported with the first generation of
FLT3 inhibitors, including lestaurtinib (CEP-701), midostaurin (PKC-412) and sorafenib.
Second generation FLT3 inhibitors such as quizartinib (AC220) are currently under
investigation with promising results. However, the hematologic toxicity of AC220 will
likely present a major limitation in evaluating AC220 combined with standard or high-dose
chemotherapy.
Ponatinib (AP24534) is a third generation tyrosine kinase inhibitor targeting the BCR-ABL
tyrosine kinase domain. Ponatinib was rationally designed with an extensive network of
optimized molecular contacts and triple bonds to accommodate the T315I mutation, a major
cause of resistance to tyrosine kinase inhibitors in chronic and advanced phase chronic
myelogenous leukemia (CML). Ponatinib also inhibits SRC (IC50: 5.4 nM) and members of the
VEGFR, FGFR, and PDGFR families of receptor tyrosine kinases (O'Hare T, Cancer Cell
2009). Despite low activity against FLT3 based on the IC50 value (FLT3 IC50: 12.6 nM
compared to BCR IC50: 0.37 nM), ponatinib has recently been reported to have significant
cellular activity against the MV4-11 cell line which harbors an FLT3-ITD activating
mutation. Ponatinib-induced apoptosis was maximal at 10 nM in vitro and a single dose of
5 and 10 mg/kg had a strong inhibitory effect in vivo in mice bearing MV4-11 xenografts.
Primary blast cells from 4 FLT3-ITD AML patients were also tested and ponatinib reduced
their viability (IC50: 4 nM) whereas no activity was shown on FLT3-ITD-negative blast
cells (Gozgit JM et al. Mol Cancer Ther 2011).
Preliminary data from the phase I clinical trial showed that 15 mg ponatinib was
associated with a Cmax of 51.1 nM. Cmax was increased to 111 nM and 149 nM in the 30 mg
and 45 mg cohorts respectively. The trough concentrations were 55.3 nM and 61.9 nM for
the 30 mg and 45 mg doses respectively (Ariad clinical investigator's brochure, version
3). Results from the ongoing phase II trial in CML patients suggest that the
hematological toxicity profile of ponatinib is comparable with that of nilotinib or
dasatinib, both of which have been successfully combined with conventional chemotherapy.
Investigators thus aim to combine ponatinib with cytarabine in FLT3-ITD AML patients in
first complete remission.
Etablissements
| Les établissements d'Île-de-France ayant mis à jour leurs données Origine et niveau de fiabilité des données | |||||
|---|---|---|---|---|---|
| HOPITAL NOVO | VAIDA | 04/07/2024 11:05:00 | Contacter | ||
| Les établissements d'Île-de-France dont les données sont issues de ClinicalTrials.gov Origine et niveau de fiabilité des données | |||||
| AP-HP - Hôpital Henri Mondor-Albert Chenevier | Contact (sur clinicalTrials) | ||||
| AP-HP - Hôpital Necker-Enfants Malades | Contact (sur clinicalTrials) | ||||
| AP-HP - Hôpital Saint Antoine | Contact (sur clinicalTrials) | ||||
| CH DE VERSAILLES SITE ANDRE MIGNOT | Contact (sur clinicalTrials) | ||||
| Les établissements sans correspondance certaine dans le répertoire FINESS dont les données sont issues de ClinicalTrials.gov Origine et niveau de fiabilité des données | |||||
| Centre Hospitalier de Meaux - 77104 - Meaux - France | Contact (sur clinicalTrials) | ||||
| Centre Hospitalier de Valenciennes - 59322 - Valenciennes - France | Contact (sur clinicalTrials) | ||||
| Centre Hospitalier René Huguenin - 92210 - Saint Cloud - France | Contact (sur clinicalTrials) | ||||
| Chr Clemenceau - 14033 - Caen Cedex - France | Contact (sur clinicalTrials) | ||||
| CHRU Dupuytren - 87042 - Limoges cedex - France | Contact (sur clinicalTrials) | ||||
| Chu Amiens - 80054 - Amiens - France | Contact (sur clinicalTrials) | ||||
| CHU Boulogne Sur Mer - 62321 - Boulogne Sur Mer cedex - France | Contact (sur clinicalTrials) | ||||
| CHU d'Angers - 49033 - Angers - France | Contact (sur clinicalTrials) | ||||
| CHU de Besançon - 25030 - Besançon - France | Contact (sur clinicalTrials) | ||||
| CHU de Dijon - 21079 - Dijon - France | Contact (sur clinicalTrials) | ||||
| CHU de Nîmes - 30029 - Nîmes - France | Contact (sur clinicalTrials) | ||||
| CHU Nice, Hôpital Archet 1 - 06202 - Nice cedex 3 - France | Contact (sur clinicalTrials) | ||||
| Dr Abdelaziz CHAIB - 13600 - Aix-en-Provence - France | Contact (sur clinicalTrials) | ||||
| Dr Arnaud PIGNEUX - 33604 - Pessac - France | Contact (sur clinicalTrials) | ||||
| Dr Christian RECHER - 31000 - Toulouse - France | Contact (sur clinicalTrials) | ||||
| Dr Edouard RANDIAMALALA - 64100 - Bayonne - France | Contact (sur clinicalTrials) | ||||
| Dr Emilie LEMASLE - 76000 - Rouen - France | Contact (sur clinicalTrials) | ||||
| Dr Jacques DELAUNAY - 44000 - Nantes - France | Contact (sur clinicalTrials) | ||||
| Dr Laurence SANHES - 66000 - Perpignan - France | Contact (sur clinicalTrials) | ||||
| Dr Mario OJEDA-URIBE - 68000 - Mulhouse - France | Contact (sur clinicalTrials) | ||||
| Dr Réda GARIDI - 02100 - Saint-Quentin - France | Contact (sur clinicalTrials) | ||||
| Dr Regis COSTELLO - 13000 - Marseille - France | Contact (sur clinicalTrials) | ||||
| Dr Stéphanie HAÏAT - 91100 - Corbeil-essonnes - France | Contact (sur clinicalTrials) | ||||
| Dr Thorsten BRAUN - 93000 - Bobigny - France | Contact (sur clinicalTrials) | ||||
| Hôpital Claude Huriez - 59037 - Lille cedex - France | Contact (sur clinicalTrials) | ||||
| Hôpital d'Instruction des Armées PERCY - 92141 - Clamart - France | Contact (sur clinicalTrials) | ||||
| Hôpital Edouard Herriot - 69437 - Lyon cedex 03 - France | Contact (sur clinicalTrials) | ||||
| Hôpital La Pitié Salpêtrière - 75013 - Paris - France | Contact (sur clinicalTrials) | ||||
| Hôpital Saint Louis - 75010 - Paris - France | Contact (sur clinicalTrials) | ||||
| Hôpital VICTOR DUPOUY - 95107 - Argenteuil - France | Contact (sur clinicalTrials) | ||||
| Institut de Cancérologie de la Loire - 42270 - Saint-Priest-en-Jarez - France | Contact (sur clinicalTrials) | ||||
| Marc BERNARD - 35000 - Rennes - France | Contact (sur clinicalTrials) | ||||
Critères
Tous
Inclusion Criteria:
1. a. Patients aged 18 to 55-60 years: Cohort A b. Patients aged 55-60 to 70 years:
Cohort B
2. Signed informed consent
3. Acute myeloid leukemia in first complete remission
4. Platelets ≥ 100 Giga/l; Neutrophils ≥ 1 Giga/l
5. Intermediate risk karyotype with FLT3-ITD activating mutant detected at diagnosis
(mutant FLT3/wild-type allelic ratio higher than 10%) (appendix 16)
6. Induction with intensive chemotherapy, dose dense sequential induction or 3 + 7 like
regimen (daunorubicin or idarubicin) for Cohort A and inclusion in the ALFA backbone
for cohort B.
7. Pancreatic functions within the normal range
8. AST or ALT less or equal to 2.5 fold upper normal range, bilirubin less or equal to
1.5 fold upper normal range
9. Serum creatinine less or equal to 1.5 fold upper normal range
10. Two planned consolidation courses with high-dose cytarabine (HDAC, Cohort A) or
intermediate dose cytarabine (IDAC, Cohort B).
1. a. Patients aged 18 to 55-60 years: Cohort A b. Patients aged 55-60 to 70 years:
Cohort B
2. Signed informed consent
3. Acute myeloid leukemia in first complete remission
4. Platelets ≥ 100 Giga/l; Neutrophils ≥ 1 Giga/l
5. Intermediate risk karyotype with FLT3-ITD activating mutant detected at diagnosis
(mutant FLT3/wild-type allelic ratio higher than 10%) (appendix 16)
6. Induction with intensive chemotherapy, dose dense sequential induction or 3 + 7 like
regimen (daunorubicin or idarubicin) for Cohort A and inclusion in the ALFA backbone
for cohort B.
7. Pancreatic functions within the normal range
8. AST or ALT less or equal to 2.5 fold upper normal range, bilirubin less or equal to
1.5 fold upper normal range
9. Serum creatinine less or equal to 1.5 fold upper normal range
10. Two planned consolidation courses with high-dose cytarabine (HDAC, Cohort A) or
intermediate dose cytarabine (IDAC, Cohort B).
1. Acute promyelocytic leukemia
2. Transformation of myeloproliferative or myelodysplastic syndromes
3. Known central nervous system involvement
4. Uncontrolled bacterial, viral or fungal infection
5. Other active malignancy
6. Previous episode of pancreatitis
7. Hypertriglyceridemia > 4.5 g/L
8. Lipase > 1.5 × ULN, amylase > 1.5 x ULN not related to leukemia
9. QTc > 470 ms (Bazett formula, see Appendix 1)
10. Patients at high or very high risk of cardiovascular disease with any of the
following f) Established cardiovascular disease
- Cardiac disease:
- Congestive heart failure greater than class II NYHA or
- Left ventricular ejection fraction (LVEF) < 50% or
- Unstable angina (anginal symptoms at rest) or
- New onset angina (began within the last 3 months) or
- Myocardial infarction, coronary/peripheral artery disease, congestive
heart failure, cerebrovascular accident including transient ischemic
attack within the past 12 months or
- History of thrombolic or embolic events
- Arrhythmias
- Any history of clinically significant cardiac arrhythmias requiring
anti-arrhythmic therapy.
g) Diabetes Mellitus untreated or not equilibrated with therapy h) Arterial
Hypertension,
-
- Uncontrolled hypertension defined as systolic blood pressure greater than
140 mmHg or diastolic pressure greater than 90 mmHg, despite optimal
medical management and optimal measurement
(http://www.has-sante.fr/portail/display.jsp?id=c_272459)
-
- Any history of hypertension with
- Hypertensive encephalopathy
- Posterior leucoencephalopathy
- Aortic or artery dissection i) Familial dysplipidemia. j) Taking
medications that are known to be associated with Torsades de Pointes (see
Appendix 11)