Informations générales (source: ClinicalTrials.gov)
Replicative Stress in Hair Follicle Stem Cells and Pathogeny of Hidradenitis Suppurativa
Interventional
N/A
Assistance Publique - Hôpitaux de Paris (Voir sur ClinicalTrials)
juin 2022
décembre 2029
14 septembre 2025
Hidradenitis Suppurativa is a recurrent chronic inflammatory follicular occlusive disease
affecting hair follicles. HS is notoriously difficult and challenging to treat with a
high morbidity impact and could be classified as an unmet medical need with no efficient
therapeutic options.
Objective:
Investigators previously showed that Outer Root Sheath Cells (ORS) isolated from hair
follicle of HS patients (HS-ORS) have a pro-inflammatory phenotype and secrete
spontaneously IP-10 and RANTES. To identify the mechanisms involved in the
pro-inflammatory phenotype of HS-ORS, investigators performed a transcriptomic analysis
in healthy and HS patients. This revealed: (i) an IFN signature, (i) a dysregulation of
genes involved in cell proliferation and differentiation, and (iii) an upregulation of
DNA damage response and cell cycle G2/M checkpoint pathways in HS-ORS. These findings
support the notion that, in HS patients, a perturbation of HF-SC homeostasis leading to
an increased proliferation induces a replicative stress and an accumulation of
cytoplasmic ssDNA, stimulating IFN synthesis through IFI16-STING pathway. Interestingly,
replicative stress in ORS were present in some but not all patients with Hidradenitis
Suppurativa. The goal of study is to determine replicative stress in ORS in a large
cohort of HS patients.
Method Patients will be enrolled in the Mondor Dermatology department, during routine
care. A dermatologist will check all inclusion and exclusion criteria with the technical
support of a research technician of the Henri Mondor Clinical Investigation Center.
Medical history, clinical data, comorbidities and concomitant therapies will be
prospectively recorded in a dedicated case report form. Skin biopsies will be performed
in perilesional zone rich in hair follicles. mRNA will be extracted from freshly isolated
hair follicle cells and some slides will be prepared and stored at -80°C to perform
immunohistochemistry analysis on freshly isolated hair follicle cells. PBMC and serum
will be collected.
All these biological samples will allow us to quantify the replicative stress in HS-ORS
of each patient, and to quantify several cytokine of interest : IFN de type 1, IL-17,
IL-6, TNF-α, IL-10
This study will allow investigators to evaluate the rate of patients with replicative
stress in hair follicle stem cells in Hidradenitis Suppurativa. The investigator will
also determine whether HF-SC replication stress correlates with clinical characteristics
and/or with clinical course and/or comorbidities.
Etablissements
| Les établissements d'Île-de-France ayant mis à jour leurs données Origine et niveau de fiabilité des données | |||||
|---|---|---|---|---|---|
| AP-HP Assistance publique - Hôpitaux de Paris | 27/12/2025 07:42:10 | Contacter | |||
| AP-HP - Hôpital Henri Mondor-Albert Chenevier | |||||
| AP-HP - Hôpital Lariboisiere-Fernand Widal | |||||
| AP-HP - Hôpital Saint Louis | |||||
Critères
Tous
Inclusion Criteria:
- Patients referred to the consultation of dermatology of Henri Mondor Hospital
- Diagnosis of Hidradenitis Suppurativa
- Age> 18 y.o.
- Affiliation to a social security system
- Patients referred to the consultation of dermatology of Henri Mondor Hospital
- Diagnosis of Hidradenitis Suppurativa
- Age> 18 y.o.
- Affiliation to a social security system
- Patient under tutorship or curatorship
- Breastfeeding or pregnant women
- Refusal to sign the consent letter
- Patients benefiting from State Medical Aid